陈加俊医生的科普号

目的： 探讨一种新的肿瘤抑素对C6脑神经胶质瘤细胞作用的影响。方法：采用MTT法检测C6脑神经胶质瘤细胞在不同时间段(12h、48h、72h)细胞增殖活性，流式细胞术检测细胞周期及Western blot法检测细胞周期蛋白-D1蛋白表达的水平。结果：新的肿瘤抑素对C6脑神经胶质瘤细胞具有明显的抑制作用，并在48小时作用最为明显。新的肿瘤抑素(2000g/mL)治疗组具有明显抑制细胞增殖；G0/G1期细胞显著升高，S期细胞数减少，与空白对照组及新的肿瘤抑素(1000L/mL)治疗组比较均有统计学意义(P<0.05)。Western Blot检测结果表明，随着新的肿瘤抑素的浓度增加，细胞周期蛋白-D1蛋白的表达水平逐渐降低；空白对照组分别与新的肿瘤抑素不同治疗组及卡莫西汀组比较均有具有显著性差异(P<0.05)。结论：新的肿瘤抑素可能通过调节细胞周期蛋白-D1蛋白的表达，细胞阻滞在S期，诱导细胞凋亡，发挥抗肿瘤细胞生长的作用。

Dachuanxing prescription, originated from An Elucidation of Formulas of Liu Wansu in Jin Dynasty, is composed of ligusticum chuanxiong and gastrodia elata; it can active blood and disperse stagnation, suppress hyperactive liver for calming endogenous wind and activate meridians to stop pain; it is mainly used to cure migraine clinically and is a classical Chinese herbal medicine compound preparation. [1]In this study, the optimum extraction process of nasal mucosa gelata for curing headache is selected and further in-depth study is done on the physicochemical properties and pharmacological effects of prescription drug. The gelata has good curative effect on headache with small side effect and few allergies after clinical trials for many years. The ligusticum Chuanxiong is the rhizome of umbelliferae ligusticum Chuanxiong that can promote qi to activate blood and dispel wind and relieve pain; its index and active ingredient is mainly ferulic acid. The gastrodia elata is the dry tuber of gastrodia elata BL with the major active ingredients of gastrodin, p-Hydroxybenzylalcohol, parahydroxybenzaldehyde, vanillyl alcohol and other phenolic compounds. The traditional Chinese medicine believes that the gastrodia elata, which is sweet, mild-natured and that can enter into liver meridian, can calm the frightened and tranquilize, and it can be used to cure light-headedness, headache, numbness of the limbs, insomnia, infantile convulsion and grand mal. Dachuanxiong gelata used in this experiment is made by the research group.

it is accidentally found by the author that a squeezing sign similar to Babinski sign near the bottom between the 4th and 5th metatarsal bone while pressing the acupoint of diseased toes of the patients with cerebral infarction according to traditional Chinese medicine; then a contrast study of the squeezing sign and Babinski sign and other common equivalent signs is conducted.

鼻粘膜给药止头痛属纯中药制剂，主要用于治疗头痛。“川芎方”，由金元时代著名医家刘完素创立，方中药物川芎可活血行气、天麻能熄风止痛，二者合用对瘀血阻滞、风阳上扰型偏头痛有良好的治疗效果。该方既简单有疗效确切。依据处方中各药材的理化性质，均可以按照醇提的方法对有效成分进行提取。经大量文献检索及专利查找，以乙醇的体积分数、提取次数和提取时间作为考察条件，但通过对处方药材理化性质及药理作用的进一步深入研究，经反复试验验证，证实了提取次数对其提取率有显著影响。综上，确定该实验的考察因素，进行正交实验考察，筛选出指标成分天麻素提取率高的工艺条件。优选鼻粘膜给药止头痛凝胶剂的最佳提取工艺。采用正交试验方法，以天麻素含量为指标，筛选鼻粘膜给药止头痛凝胶剂最佳提取工艺条件。鼻粘膜给药止头痛凝胶剂最佳提取工艺条件为乙醇。优选得到的工艺稳定可行，能显著提高阿魏酸的含量。

维生素B12缺乏通过多种机制损害神经功能。首先，维生素B12在体内介导两种重要的酶促反应，一是甲基丙二酰CoA转化为琥珀酰CoA，另一为Hcy转化为蛋氨酸。在后一反应中，甲钴胺作为蛋氨酸合酶的辅因子，将5甲基四氢叶酸的甲基转给Hcy使形成蛋氨酸。腺苷基化的蛋氨酸（SAM）作为甲基的供体，参与诸多重要物质的甲基化过程，包括DNA、RNA、蛋白质、髓磷脂和诸多神经递质等。一旦体内维生素B12缺乏，则SAM生成受阻，导致严重的代谢障碍，引起神经髓鞘形成障碍和脱失等神经病变。其次，因转化受阻而积聚的Hcy通过刺激N-甲基-D-天(门)冬氨酸（NMDA）受体和激活凋亡相关蛋白Bax和p53等机制产生细胞毒性作用，损害神经功能。第三，蛋氨酸合成障碍使甲基四氢叶酸不能转化为四氢叶酸，从而导致亚甲基四氢叶酸生成减少。后者是RNA前体脱氧尿苷酸转化为DNA前体脱氧胸苷酸的重要辅因子，其减少造成DNA合成受阻，使神经功能障碍，以上即所谓的“叶酸陷阱”假说。最后，维生素B12缺乏使S-腺苷同型半胱氨酸（SAH）不易转化而积聚，后者是多种甲基转移酶的强力抑制剂，其数量减少势必导致甲基化过程受阻，影响神经细胞的正常代谢［4］。

In the paper, WPU nanometer titanium dioxidecomposite is made mainly of polypropylene glycol (PPG), 2, 4-toluenediisocynate (TDI), nanometer titanium dioxide (TiO2), oleic acid(OA), dimethylolpropionic acid (DMPA), dibutyltin dilaurate (DBTDL), triethylamine(TEA), polypropylene glycol phosphate (PPG-P). Feature of nano particle is analyzed andtested by scanning electron microscope; Fourier infrared spectrum ofnanoparticle and composite is tested by fourier infrared spectrometer underroom temperature; thermal property test is conducted to composite by thermogravimetricanalyzer; XRDtest is conducted to nanoparticle and compound thin-film material by X-raydiffractometer; mechanical property of composite is tested by universalstretcher, proving that titanium dioxide does not change structure of WPU andtitanium dioxide keeps the original crystal structure instead of beingdestroyed. Thermogravimetric analysis shows that titanium dioxideraises thermal stability of composite better; mechanical properties prove thatincrease of 2wt% nanometer titanium dioxide can raise mechanical properties ofWPU. Titanium dioxide has extensive application prospect in production and lifebecause it has optical activity and can raise antibiotic property of WPU.Polyurethane isthe floorboard of the polymer of repetitive carbamic acid esters (-NHCOO -) andcan adjust its physical and chemical properties greatly through proportionbetween optimal soft and hard sections [1-4]. Polyurethane material is calledan ideal biomaterial and a kind of important biomedical material [5] . we need to raise antibiotic property of polyurethaneand expand its application in medical field [6].

目的:探讨既有中枢神经又有周围神经脱髓鞘疾病的临床特点,以提高对此类疾病的认识.方法:对此类病例的临床资料进行回顾性分析.结果:病人症状、体征都比较弥散,中枢神经系统脱髓鞘改变又伴有周围神经损伤.因此,很难用现有疾病名称来完整解释此类现象.结论:需要重新认识中枢神经合并周围神经脱髓鞘疾病,这对今后的临床工作有很大的指导意义.

目的:研究脑梗死患者肱动脉血流介导的舒张功能(FMD),探讨FMD与颈动脉粥样硬化的相关性,为脑梗死的早期诊断、病情进展、治疗效果评估与预后判断提供参考.方法:60例健康体检者为对照组,2007～2009年门诊及住院的急性脑梗死患者60例为脑梗死组.利用超声检测脑梗死组及对照组肱动脉FMD,颈动脉IMT,观察有无斑块形成.结果:①急性脑梗死组肱动脉FMD较对照组显著降低.②有颈动脉斑块形成者肱动脉FMD较IMT增厚者降低,二者存在差异.③有偏侧肢体瘫痪的急性脑梗死患者双侧肱动脉FMD无显著性差异.结论:①急性脑梗死患者FMD明显受损,并且随颈动脉粥样硬化程度的加重而加重.②临床上肱动脉FMD检测可以评估血管的功能改变,而颈部血管超声测量反映的是血管的形态学改变,二者可互为补充来评价动脉粥样硬化程度.③急性脑梗死患者肢体瘫痪不会影响肱动脉血流介导的扩张.

n the paper, the best extraction technology of nasal mucosa dosingbromo gels is preferably selected. Orthogonal test is applied to screen the besttechnological condition for extracting nasal mucosa dosing bromo gels withcontent of ferulic acid and gastrodin as the indexes; it is figured out thatthe best technological condition is ethanol; the optimal technology is stableand feasible and can raise content of ferulic acid remarkably. Nasal mucosadosing bromo gels belongs to a kind of pure Chinese materia medica preparationand is mainly used to cure headache. Hemlockparsley prescription is created byfamous medical scientist Liu Wansu in Jin and Yuan Dynasties; in theprescription, hemlock parsley can invigorate the circulation of blood andpromote the circulation of the whole body; gastrodia elata can relievedizziness and pain; both have good therapeutic effects to blood stasis, retardationand migraine [1]. We can say it is simple with excellent curative effect.The prescriptionconsists of hemlock parsley and gastrodia elata[2] and has good curative effect toheadache with faint toxic and side effects via several years of clinical trial;besides, anaphylaxis is rarely seen to patients. Its effective elements can beextracted by water extraction according to physical and chemical properties ofmedicines in the prescription. It is tested in experiments that the indexelement of ferulic acid is lower than that of ethanol purely acquired by waterextraction [3].In plenty ofdocument retrievals and patent searches, technological conditions forextracting ferulic acid, volume fraction, extraction times and extraction time ofethanol are not taken as investigation conditions. It has been proved throughdeeply researches and repeated experiments of physical and chemical propertiesand pharmacological effects of medicines in the prescription that extract timesremarkably influence extraction efficiency. Above all, investigation factors are conformed; orthogonal testis conducted and technological conditions for extracting ferulic acid arescreened.

神经系统功能定位学说认为，脑的特定部位支配特定的功能，该部位损害即发生特定的功能障碍。以前在脑细胞不可再生理论的影响下，一旦出现脑梗死就认为是不可恢复的，从而造成了神经病学重诊断轻治疗的局面，认为没有更多的有效治疗手段。然而，基础研究以及临床实践现证明脑梗死后功能是有可能或有条件恢复的。目前人们对脑可塑性的认识逐渐从否认、争议发展到基本认同。脑可塑性研究还不多，针刺影响的研究极少。脑可塑性是指脑组织具有在结构和功能上自身修复的能力[1]，是临床治疗的重要基础[2]。我们观察了脑缺血损伤后标志缺血损伤程度，轴突树突修复，突触可塑性及神经再生的一系列指标的动态变化，旨在研究脑梗死后脑可塑性的物质基础和机制及与行为学的关系，观察了针刺的影响，并运用于临床治疗。在临床上我们将脑可塑性理论与神经康复理论相结合，形成针刺促通术的理论和技术即针刺时不但要结合经络理论，更要注意肌张力的变化-这一本质区别及神经肌肉的解剖，应用于临床，观察了疗效。